By Alton Meister

ISBN-10: 0470122668

ISBN-13: 9780470122662

ISBN-10: 0470648783

ISBN-13: 9780470648780

Advances in Enzymology and comparable components of Molecular Biology is a seminal sequence within the box of biochemistry, supplying researchers entry to authoritative experiences of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, delivering an unequalled view of the old improvement of enzymology. The sequence bargains researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic technique, and their software in either the laboratory and undefined. each one quantity within the sequence good points contributions through prime pioneers and investigators within the box from around the globe. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide selection of themes and lengthy old pedigree, Advances in Enzymology and comparable components of Molecular Biology can be utilized not just by means of scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally through any scientist attracted to the invention of an enzyme, its houses, and its purposes.

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Pathways in the synthesis of lecithin and triglycerides. microsome-containing supernatant of the liver was added. This was shown t o be due to the fact that a-glycerophosphate was an intermediate in the reaction, and that the supernatant contained glycerophosphokinase. The latter enzyme was purified from this fraction of the liver by Bublitz and Kennedy (306). Kornberg and Pricer (307) also studied the formation of phosphatidic acid in liver, but in a different manner. They found that either palmitoyl-CoA or palmitate plus ATP and CoA were converted to phosphatidic acid in the presence of a-glycerophosphate and Iiver enzymes.

These results were in marked contrast to the findings of Popjak and Tietx (301), and of Jmgdon (135). These workers reported that the soluble fraction of mammary gland and of rat liver were able to catalyze the incorporation of acetate into fatty acids in the absence of mitochondria. Brady, Mamoon, and Stadtman (302) subsequently stated that the incorporation of acetate into fatty acids required only the soluble fraction of pigeon liver if citrate was added to the incubation medium. However, Gurin’s group [cf.

Nuclei have also been reported to increase phosphate esterification during the oxidation of oxalacetate-pyruvate (126) or a-ketoglutarate (1 74) by mitochondria. The phosphorylnthg efficiency was again lower with the combined fractions than with the mitochondri:i alone. Stern and Timonen (175) have challenged the conclusion that nuclei stimulate oxidative phosphorylation on the basis of experiments using glutamate as substrate which showed (1) that neither oxidation nor phosphorylation was proportional to mitochondria1 concentration, (2) that nuclear stimulation of oxidative phosphorylation was due to elimination of this dilution effect, and (3)that heated nuclei or albumin also stimulated oxidative phosphorylation.

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Advances in Enzymology and Related Areas of Molecular Biology, Volume 21 by Alton Meister


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